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Status |
Public on Jun 20, 2013 |
Title |
scrambled transfected MEG-01, biological replicate 2 |
Sample type |
RNA |
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|
Source name |
Meg-01 cells, no treatment
|
Organism |
Homo sapiens |
Characteristics |
cell line: Meg-01 cells genotype/variation: control
|
Treatment protocol |
Cultured MEG-01 cells were transfected with 200nM of upregulated pre-miRNA 1225-3p or negative control and left for incubation for 24 hours
|
Growth protocol |
The cells were subcultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) and penicillin/streptomycin under 5% CO2 at 37 degrees Celsius.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using miRNeasy mini kit (Qiagen) according to the manufacturer's instructions.
|
Label |
Cy3
|
Label protocol |
Cy3 labeled RNA was prepared according to the standard Agilent protocol from 100ng of total RNA
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Hybridization protocol |
Labeled RNA was hybridized for 17 hr at 65C on Agilent 8X60K microarray. Arrays were washed according to the manufacturer's protocol.
|
Scan protocol |
Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505B) using one color scan setting for 8x60K array slides
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Data processing |
The scanned images were analyzed with Feature Extraction Software 10.7.3.1 (Agilent) using default parameters. LincRNAs have been excluded from the normalization. Positive and negative controls are also removed.
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Submission date |
Oct 15, 2012 |
Last update date |
Jun 20, 2013 |
Contact name |
Shilpa Jain |
E-mail(s) |
shilpa.jain3@chp.edu
|
Organization name |
Uinversity of Pittsburgh
|
Street address |
200 Lothrop Street
|
City |
Pittsburgh |
State/province |
PA |
ZIP/Postal code |
15213 |
Country |
USA |
|
|
Platform ID |
GPL14550 |
Series (2) |
GSE41573 |
Gene expression data in Meg-01 cells following transfection with platelet-enriched microRNA-1225-3p |
GSE41575 |
Role of platelet micrornas in sickle cell disease |
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