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Sample GSM1023794 Query DataSets for GSM1023794
Status Public on Oct 17, 2015
Title 28_12w_1
Sample type RNA
 
Source name Peripheral Blood, 12 week
Organism Homo sapiens
Characteristics tissue: whole blood
gender: male
time point: after intake
treatment: Chlorella
Treatment protocol A peripheral blood sample was collected in a PAXgene blood RNA tube (BD, City, NJ, USA), left at room temperature for 2 hours after sufficient mixing, and then stored at −20℃.
Extracted molecule total RNA
Extraction protocol The stored tube was used for total RNA extraction with the PAXgene Blood miRNA Kit (QIAGEN, City, CA, USA). RNA was quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer.
Label Cy3
Label protocol Cyanine 3-labeled cRNA was prepared from 100 ng total RNA using the Low Input Quick Amp Labeling Kit according to the manufacturer’s protocol.
 
Hybridization protocol Agilent one-color gene expression hyb/wash protocol.
Scan protocol Microarrays were scanned using a G2505C DNA Microarray Scanner.
Description Gene expression profile in the peripheral blood after Chlorella intake.
Data processing The images were converted to numerical values by Feature Extraction Software ver. 10.7.3.1. Data analysis used GeneSpring GX v11.5 (Agilent Technologies) with which inter-array normalization was done using the 75th percentile.
 
Submission date Oct 23, 2012
Last update date Oct 17, 2015
Contact name Michie Kobayashi
E-mail(s) kobayashi@dna-chip.co.jp
Phone +81-45-500-5211
Organization name DNA Chip Research Inc.
Street address 1-1-43, Suehirocho,
City Tsurumi-ku,
State/province Yokohama
ZIP/Postal code 230-0045
Country Japan
 
Platform ID GPL14550
Series (1)
GSE41767 Chlorella ingestion and suppression of resistin gene expression in borderline diabetics: a randomized, placebo-controlled study

Data table header descriptions
ID_REF
VALUE Data analysis used GeneSpring GX v11.5 (Agilent Technologies) with which inter-array normalization was done using the 75th percentile.

Data table
ID_REF VALUE
GE_BrightCorner 0.15610409
DarkCorner 0.011599064
A_23_P326296 -0.11149311
A_24_P287941 0.17461872
A_24_P325046 -0.13446784
A_23_P200404 -0.3437767
A_19_P00800513 0.6821127
A_23_P15619 1.3424392
A_33_P3402354 -0.39906287
A_33_P3338798 -0.41541338
A_32_P98683 0.02925682
A_23_P137543 0.26005054
A_19_P00803040 1.4933114
A_23_P117852 -0.68565536
A_33_P3285585 -0.43929768
A_24_P328231 -0.24587989
A_33_P3415668 -0.4030261
A_23_P73609 -0.2519436
A_24_P186124 -0.55134296
A_23_P369983 -0.1013298

Total number of rows: 42545

Table truncated, full table size 1037 Kbytes.




Supplementary file Size Download File type/resource
GSM1023794_28_12w_1.txt.gz 12.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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