|
| Status |
Public on Mar 08, 2013 |
| Title |
G166_GSC line_3 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Glioma Stem Cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: G166
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega).
|
| Label |
Cy3
|
| Label protocol |
1µg of genomic DNA was labeled according to the Agilent guidelines (www.agilent.com). Sample DNA was labeled with Cy3 and the Human Genomic DNA: Male (Promega), used as reference DNA, was labeled with Cy5.
|
| |
|
| Channel 2 |
| Source name |
Human Genomic DNA: Male (Promega)
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: Genomic DNA comes from multiple anonymous donors.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega).
|
| Label |
Cy5
|
| Label protocol |
1µg of genomic DNA was labeled according to the Agilent guidelines (www.agilent.com). Sample DNA was labeled with Cy3 and the Human Genomic DNA: Male (Promega), used as reference DNA, was labeled with Cy5.
|
| |
|
| |
| Hybridization protocol |
Hybridization was performed on Agilent-014950 Human Genome CGH microarray 4x44K, following manufacturer's instructions (www.agilent.com for details).
|
| Scan protocol |
Briefly, the arrays were scanned at 2μm resolution using Agilent microarray scanner (Agilent Technologies, Santa Clara, CA, USA).
|
| Data processing |
Data were analyzed using Feature Extraction v10.7 and Agilent Genomic Workbench v5.0 softwares. The Aberration Detection Method 2 (ADM2) algorithm was used to compute and assist the identification of aberrations for each sample.
|
| |
|
| Submission date |
Oct 26, 2012 |
| Last update date |
Mar 08, 2013 |
| Contact name |
Simona Baronchelli |
| Organization name |
University of Milan-Bicocca
|
| Street address |
Via Cadore 48
|
| City |
Monza |
| ZIP/Postal code |
20900 |
| Country |
Italy |
| |
|
| Platform ID |
GPL8841 |
| Series (1) |
| GSE41875 |
Genome variation profiling of Glioma Stem Cell lines |
|
