|
| Status |
Public on Feb 01, 2013 |
| Title |
Reh cells_bortezomib_12h |
| Sample type |
RNA |
| |
|
| Source name |
Reh cells, treatment with bortezomib, 12h
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Reh
tissue: leukoblasts
|
| Treatment protocol |
Reh cells were seeded at a denstity of 1x10E06 cells/ml in 12 well plates with corresponding drugs added from 100x stock solutions to achieve final concentrations.
|
| Growth protocol |
RPMI 1640 Medium, 10%FCS, 1%Pen/Strep, 37°C, 5%CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
mRNA was extracted by using the RNeasy Kit (Quiagen).
|
| Label |
cy3
|
| Label protocol |
Labeling was performed according to the protocol using the Agilent Low Input Quick Amp Labeling Kit (Agilent)
|
| |
|
| Hybridization protocol |
Hybridization was performed according to the Agilent 60-mer oligo microarray processing protocol using the Agilent Gene Expression Hybridization Kit (Agilent)
|
| Scan protocol |
Fluorescence signals of the hybridized Agilent Microarrays were detected using Agilent’s Microarray Scanner System (Agilent).
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 9.1 (Agilent). Further data analysis was performed using the Partek® Genomics Suits software, version 6.5 beta © 2009 (Partek Inc., USA).
|
| |
|
| Submission date |
Oct 31, 2012 |
| Last update date |
Feb 02, 2013 |
| Contact name |
Shabnam Shalapour |
| E-mail(s) |
shabnam.shalapour@charite.de
|
| Organization name |
Charité - Universitätsmedizin Berlin
|
| Street address |
Augustenburger Platz 1
|
| City |
Berlin |
| ZIP/Postal code |
13353 |
| Country |
Germany |
| |
|
| Platform ID |
GPL13607 |
| Series (1) |
| GSE41951 |
Gene expression profile of the human leukemia cell line Reh after treatment with bortezomib, valproic acid or a combination of both. |
|
