|
Status |
Public on Mar 05, 2013 |
Title |
Control fibroblasts IFNa, biological rep2 |
Sample type |
RNA |
|
|
Source name |
Dermal fibroblasts from control, IFNa
|
Organism |
Homo sapiens |
Characteristics |
genotype/variation: wild type cell type: primary dermal fibroblasts treatment: interferon-alpha (IFNa)
|
Treatment protocol |
Cells were either treated with 1,000 U/ml interferon-alpha for 10 hrs or given a mock treatment.
|
Growth protocol |
Patient (V:3) and control fibroblasts were cultured in DMEM containing 10% FCS in 24-well plates.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total cellular RNA was isolated using the QIAgen RNeasy Plus kit protocol.
|
Label |
biotin
|
Label protocol |
Standard Affymetrix labeling procedure.
|
|
|
Hybridization protocol |
Standard Affymetrix hybridization and wash procedure FS450_0002.
|
Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner G3000 7G using the AGCC software tool.
|
Description |
Gene expression data from control fibroblasts treated with 1,000U/ml interferon-alpha for 10h.
|
Data processing |
R/Bioconductor using RMA normalization, non-specific expression level filter of 50 applied and empirical Bayes statistical test with Benjamini-Hochberg FDR adjustment performed.
|
|
|
Submission date |
Oct 31, 2012 |
Last update date |
Mar 05, 2013 |
Contact name |
Paul Dickinson |
E-mail(s) |
Paul.Dickinson@ed.ac.uk
|
Phone |
0131 2426287
|
Organization name |
University of Edinburgh
|
Department |
Division of Infection and Pathway Medicine
|
Street address |
49 Little France Crescent
|
City |
Edinburgh |
ZIP/Postal code |
EH16 4SB |
Country |
United Kingdom |
|
|
Platform ID |
GPL15207 |
Series (1) |
GSE41960 |
Expression data from primary human dermal fibroblasts with STAT2 deficiency and control fibroblasts treated with interferon |
|