|
| Status |
Public on Nov 08, 2012 |
| Title |
CD34_260511_hm450 |
| Sample type |
genomic |
| |
|
| Source name |
Density centrifuged bone marrow
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: Healthy donor cells
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Bisulfite conversion of genomic DNA was performed using using the EZ DNA Methylation™ Kit (ZymoResearch, Irvine, CA, USA). Bisulphite converted DNA was amplified, fragmented and 500 ng converted DNA was hybridized to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol.
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
DNA was hybridized to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using HiScan instrument (Illumina Inc.) using standard recommended Illumina scanner setting
|
| Description |
Healthy donor cells
|
| Data processing |
Data preprocessing and methylation level extraction was performed using the Genome Studio software v2011.1 (Illumina Inc.) including the Methylation module v1.9.0 and the Illumina Genome Viewer module v1.9.0.
|
| |
|
| Submission date |
Nov 07, 2012 |
| Last update date |
Nov 08, 2012 |
| Contact name |
Christian Rohde |
| E-mail(s) |
christian.rohde@uni-heidelberg.de
|
| Organization name |
Heidelberg University
|
| Lab |
Molecular Hematology and Oncology
|
| Street address |
Im Neuenheimer Feld 410
|
| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
| |
|
| Platform ID |
GPL13534 |
| Series (2) |
| GSE42118 |
DNA methylation changes are a late event in Acute Promyelocytic Leukemia and coincide with loss of transcription factor binding (Illumina Methylation) |
| GSE42119 |
DNA methylation changes are a late event in Acute Promyelocytic Leukemia and coincide with loss of transcription factor binding |
|
