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Sample GSM1040916 Query DataSets for GSM1040916
Status Public on May 23, 2013
Title HuH-7 FLAG-TAp63 gamma-24h
Sample type RNA
 
Source name HuH-7 human hepatocellular carcinoma cells
Organism Homo sapiens
Characteristics cell transfection: TAp63gamma expressing adenovirus for 24 h
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Trizol Reagent (Invitrogen).
Label Cy3
Label protocol Total RNA (100 ng) was labeled using a Low Input Quick Amp Labeling Kit One-Color (Agilent Technologies) according to manufacturer's instruction.
 
Hybridization protocol Hybridization was performed using a Gene Expression Hybridization kit (Agilent Technologies) according to manufacturer's instruction.
Scan protocol Array was scanned with an Agilent G2565BA Microarray Scanner.
Data processing Agilent Feature Extraction Software (v 10.7) was used for background subtraction.
 
Submission date Nov 26, 2012
Last update date May 23, 2013
Contact name Yasushi Sasaki
E-mail(s) yasushi@sapmed.ac.jp
Phone 81-11-611-2111
Fax 81-11-618-3313
Organization name Sapporo Medical University
Department Cancer Research Institute
Lab Molecular Biology
Street address S-1, W-17, Chuo-ku
City Sapporo
ZIP/Postal code 060-8556
Country Japan
 
Platform ID GPL13607
Series (1)
GSE42493 Gene expression signatures in HuH7 cells transfected with super hybrid p53 version 2.

Data table header descriptions
ID_REF
VALUE background subtracted signal intensity

Data table
ID_REF VALUE
1 5.23E+04
2 3.01E+00
3 3.04E+00
4 1.62E+02
5 6.67E+02
6 7.95E+01
7 5.33E+03
8 1.55E+03
9 3.20E+00
10 1.47E+01
11 3.25E+00
12 1.89E+02
13 1.43E+03
14 6.99E+02
15 6.81E+03
16 3.32E+00
17 9.74E+01
18 3.34E+00
19 5.12E+00
20 9.70E+02

Total number of rows: 62976

Table truncated, full table size 911 Kbytes.




Supplementary file Size Download File type/resource
GSM1040916_US45103036_252800412957_S01_GE1_107_Sep09_1_3.txt.gz 12.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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