|
| Status |
Public on Mar 03, 2014 |
| Title |
rhesus_monkey_lung |
| Sample type |
SRA |
| |
|
| Source name |
lung
|
| Organism |
Macaca mulatta |
| Characteristics |
tissue: lung
gender: male
library type: strand-specific, dUTP protocal
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol method
Poly (A)-positive RNA was purified with the Dynabeads mRNA purification kit (Invitrogen) from 5μg of total RNA with high quality (RNA integrity number >7.5), following the manufacturer's instructions. A strand-specific RNA-Seq library preparation on the basis of the incorporation of deoxy-UTP was performed.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
RNA-Seq data were mapped to genome (rheMac2) tophat (v1.2.0)
RPKM was calculated as previously described
Genome_build: rheMac2
Supplementary_files_format_and_content: Table format. The gene and transcript ID, RPKM, reads number mapped to this gene, the length of exon and the position were listed in order.
|
| |
|
| Submission date |
Dec 11, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Jia-Yu Chen |
| E-mail(s) |
jiayujiayu2008@163.com
|
| Organization name |
Peking University
|
| Department |
Institute of Molecular Medicine
|
| Lab |
Lab of Genomic Medicine
|
| Street address |
No.5 Yiheyuan Road Haidian District
|
| City |
Beijing |
| ZIP/Postal code |
100871 |
| Country |
China |
| |
|
| Platform ID |
GPL14954 |
| Series (1) |
| GSE42857 |
Transcriptome study in rhesus monkey |
|
| Relations |
| SRA |
SRX209572 |
| BioSample |
SAMN01830805 |
