|
Status |
Public on Jan 01, 2015 |
Title |
Expression in colon mucosa 395 |
Sample type |
RNA |
|
|
Source name |
colon mucosa 395
|
Organism |
Homo sapiens |
Characteristics |
gender: Female age: 12 sample type: Colon mucosa disease state: Ulcerative colitis
|
Treatment protocol |
Flash freezing of biopsy samples on dry ice
|
Growth protocol |
None
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiangen QIAzol
|
Label |
biotin
|
Label protocol |
Nugen Ovation Pico WTA System V2 /Encore Biotin Module
|
|
|
Hybridization protocol |
Fluidics Protocol FS450_0002
|
Scan protocol |
Affymetrix Genechip Scanner 7G
|
Description |
Ulcerative colitis colon mucosa sample
|
Data processing |
The R Bioconductor affy package rma function was used to compute expression. The rma function performs probe specific correction of the PM probes using a model based on observed intensity being the sum of signal and noise, normalization of corrected PM probes using quantile normalization, and calculation of expression measure using median polish.
|
|
|
Submission date |
Dec 13, 2012 |
Last update date |
Jan 01, 2015 |
Contact name |
R. Alan Harris |
E-mail(s) |
rharris1@bcm.edu
|
Organization name |
Baylor College of Medicine
|
Department |
Molecular and Human Genetics
|
Street address |
1 Baylor Plaza
|
City |
Houston |
State/province |
TX |
ZIP/Postal code |
77030 |
Country |
USA |
|
|
Platform ID |
GPL15207 |
Series (1) |
GSE42911 |
Expression data in colon mucosa samples from individuals with ulcerative colitis and normal controls |
|