|
| Status |
Public on Jan 01, 2015 |
| Title |
Expression in colon mucosa 434 |
| Sample type |
RNA |
| |
|
| Source name |
colon mucosa 434
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Male
age: 17
sample type: Colon mucosa
disease state: normal
|
| Treatment protocol |
Flash freezing of biopsy samples on dry ice
|
| Growth protocol |
None
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiangen QIAzol
|
| Label |
biotin
|
| Label protocol |
Nugen Ovation Pico WTA System V2 /Encore Biotin Module
|
| |
|
| Hybridization protocol |
Fluidics Protocol FS450_0002
|
| Scan protocol |
Affymetrix Genechip Scanner 7G
|
| Description |
Normal colon mucosa sample
|
| Data processing |
The R Bioconductor affy package rma function was used to compute expression. The rma function performs probe specific correction of the PM probes using a model based on observed intensity being the sum of signal and noise, normalization of corrected PM probes using quantile normalization, and calculation of expression measure using median polish.
|
| |
|
| Submission date |
Dec 13, 2012 |
| Last update date |
Jan 01, 2015 |
| Contact name |
R. Alan Harris |
| E-mail(s) |
rharris1@bcm.edu
|
| Organization name |
Baylor College of Medicine
|
| Department |
Molecular and Human Genetics
|
| Street address |
1 Baylor Plaza
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL15207 |
| Series (1) |
| GSE42911 |
Expression data in colon mucosa samples from individuals with ulcerative colitis and normal controls |
|
