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Sample GSM1055772 Query DataSets for GSM1055772
Status Public on Apr 03, 2013
Title H3K79me2_MLL-AF6 rep3
Sample type SRA
 
Source name Leukemic bone marrow cells, primary
Organism Mus musculus
Characteristics background strain: B6/129 (Taconic)
chip antibody: H3K79me2
cell type: Leukemic bone marrow cells, primary
disease state: MLL-AF6 positive leukemia
chip antibody manufacturer: Abcam
chip antibody cataolog #: Ab3594
Treatment protocol Frozen leukemia bone marrow cells were thawed using standard conditions and 1 million cells were used for fixing and subsequent ChIP seq steps.
Growth protocol Cells were not grown prior to the ChIP-seq
Extracted molecule genomic DNA
Extraction protocol DNA was sheared from the fixed MLL-AF6 leukemia cells using sonication. Histone-DNA complexes enriched for H3K79me2 were isolated by immunoprecipitation with the Abcam anti-H3K79me2 specific antibody (Ab 3594)
ChIP DNA libraries were made following the Illumina ChIP-seq library preparation kit.
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina HiSeq 2000
 
Description ChIP-Seq Multiplexing using Illumina adapters
Data processing Sequence reads were aligned to human genome assembly mm9 using BWA with default parameters.
Wiggle files were generated using a using a custom pipeline.
Genome_build: mm9
Supplementary_files_format_and_content: wig
 
Submission date Dec 20, 2012
Last update date May 15, 2019
Contact name Amit Sinha
E-mail(s) amit.sinha@childrens.harvard.edu
Phone 617-582-7579
Organization name Dana-Farber Cancer Institute
Department Pediatric Oncology
Lab Armstrong Lab
Street address 44 Binney St
City Boston
State/province MA
ZIP/Postal code 02135
Country USA
 
Platform ID GPL13112
Series (2)
GSE43062 Genome wide mapping of histone 3 lysine 79 dimethylation in MLL-AF6 murine leukemias
GSE43069 MLL-AF6 leukemia
Relations
SRA SRX212166
BioSample SAMN01832294

Supplementary file Size Download File type/resource
GSM1055772_MLL-AF6-rep3.H3K79me2.endtoend.mm9.wig.gz 24.9 Mb (ftp)(http) WIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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