|
| Status |
Public on Dec 13, 2013 |
| Title |
MM1S_WCE_AT7519 |
| Sample type |
SRA |
| |
|
| Source name |
Multiple myeloma
|
| Organism |
Homo sapiens |
| Characteristics |
chip antibody: None
chip antibody catalog number: None WCE
chip antibody manufacturer: None WCE
cell line: MM1.S
duration: 6h
concentration: 2uM
drug: AT7519
barcode removed: CGTACG
|
| Treatment protocol |
Treated with AT7519 (2uM for 6 hours), Cdk9 inhibitor
|
| Growth protocol |
RPMI 1640 supplemented with 10% FCS and 1% GlutaMAX
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Whole cell extracts were sonicated to solubilize the chromatin. The chromatin extracts containing DNA fragments with an average size of 500 bp were immunoprecipitated using different antibodies. Purified immunoprecipitated DNA were prepared for sequencing according to a modified version of the Solexa Genomic DNA protocol. Fragmented DNA was end repaired and subjected to 18 cycles of LM-PCR using oligos provided by Illumina. Amplified fragments between 150 and 300bp (representing shear fragments between 50 and 200nt in length and ~100bp of primer sequence) were isolated by agarose gel electrophoresis and purified.
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
WCE_MM1S_AT7519
|
| Data processing |
Images analysis and base calling was done using the solexa pipeline.
For all samples reads were aligned to their indicated build using bowtie with parameters -e 70 -k 2 -m 2 -n 2 --best --concise. Seed length (-l) was set to read length for each dataset.
Genome_build: hg18
Supplementary_files_format_and_content: WIG files: For all samples aligned sequences were extended 150bp upstream and 0bp downstream (with respect to read strand) and allocated into 25bp bins. Counts were normalized to reads per million, and bins with at least 1 read per million are shown.
|
| |
|
| Submission date |
Jan 24, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Richard A Young |
| E-mail(s) |
young_computation@wi.mit.edu
|
| Phone |
617-258-5219
|
| Organization name |
Whitehead Institute for Biomedical Research
|
| Lab |
Young Lab
|
| Street address |
9 Cambridge Center
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platform ID |
GPL11154 |
| Series (1) |
| GSE43743 |
ChIP-Seq of transcriptional regulators in MM1S cells upon small molecule treatment. |
|
| Relations |
| SRA |
SRX219378 |
| BioSample |
SAMN01901765 |
