|
| Status |
Public on Jun 13, 2014 |
| Title |
BM-MTG16_WT |
| Sample type |
RNA |
| |
|
| Source name |
Bone marrow
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
cell type: dendritic cells
genotype/variation: wild type
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Used the Ambion WT labelling kit as per manufacturer's instructions using 200ng of starting total RNA
|
| |
|
| Hybridization protocol |
Biotinylated DNA probe was made according to the standard Affymetrix protocol
|
| Scan protocol |
Arrays were scanned according to the Affymetrix protocol
|
| Data processing |
Affymetrix manufacturer software (employing gcRMA normalization) was used to process data.
|
| |
|
| Submission date |
Jan 29, 2013 |
| Last update date |
Jun 13, 2014 |
| Contact name |
Hiyaa Singhee Ghosh |
| E-mail(s) |
hg2238@columbia.edu
|
| Organization name |
Columbia University Medical Center
|
| Street address |
701 W 168th Street
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE43874 |
Mtg16 regulates E protein activity and lineage specification in dendritic cell development (gene expression) |
| GSE43963 |
Mtg16 regulates E protein activity and lineage specification in dendritic cell development |
|
