|
Status |
Public on Jun 13, 2014 |
Title |
BM-MTG16_KO |
Sample type |
RNA |
|
|
Source name |
Bone marrow
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 cell type: dendritic cells genotype/variation: mtg16-KO
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Used the Ambion WT labelling kit as per manufacturer's instructions using 200ng of starting total RNA
|
|
|
Hybridization protocol |
Biotinylated DNA probe was made according to the standard Affymetrix protocol
|
Scan protocol |
Arrays were scanned according to the Affymetrix protocol
|
Data processing |
Affymetrix manufacturer software (employing gcRMA normalization) was used to process data.
|
|
|
Submission date |
Jan 29, 2013 |
Last update date |
Jun 13, 2014 |
Contact name |
Hiyaa Singhee Ghosh |
E-mail(s) |
hg2238@columbia.edu
|
Organization name |
Columbia University Medical Center
|
Street address |
701 W 168th Street
|
City |
New York |
State/province |
NY |
ZIP/Postal code |
10032 |
Country |
USA |
|
|
Platform ID |
GPL6246 |
Series (2) |
GSE43874 |
Mtg16 regulates E protein activity and lineage specification in dendritic cell development (gene expression) |
GSE43963 |
Mtg16 regulates E protein activity and lineage specification in dendritic cell development |
|