|
| Status |
Public on May 07, 2014 |
| Title |
10235 |
| Sample type |
RNA |
| |
|
| Source name |
Human Bronchial Epithelial Cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HBEC3KT
cell type: Human Bronchial Epithelial Cells
radiation: Fe
dose (gray): 0.5
post radiation time (hour): 12
biological replicate: 09C
technical replicate: A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with QIAGEN RNeasy Plus mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Bio-rad Experion system.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with Ambion TotalPrep RNA amplification kit with biotin UTP (Enzo) labeling
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
5139571008_B
|
| Data processing |
The data were background subtracted and normalised using MBCB algorithm
|
| |
|
| Submission date |
Feb 12, 2013 |
| Last update date |
May 07, 2014 |
| Contact name |
Lianghao Ding |
| E-mail(s) |
lianghao.ding@utsouthwestern.edu
|
| Phone |
214-648-5892
|
| Organization name |
UT Southwestern Medical Center
|
| Street address |
5323 Harry Hines Blvd
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390 |
| Country |
USA |
| |
|
| Platform ID |
GPL13376 |
| Series (1) |
| GSE44282 |
Transcriptome Profiles in Normal Human Bronchial Epithelial Cells after Exposure to γ-rays and different HZE particles |
|
