|
Status |
Public on Dec 31, 2014 |
Title |
DAOY_H3 |
Sample type |
RNA |
|
|
Source name |
DAOY cells transduced with lentivirus encoding anti-ZFX shRNA H3
|
Organism |
Homo sapiens |
Characteristics |
cell line: DAOY genotype: ZFX KD
|
Treatment protocol |
DAOY cells were transduced for 16-24 hours with lentivirus encoding shRNA targeting ZFX (H2, H3, H4) or scrambled non-targeting shRNA (SCR). Cells were allowed to recover for 48 hours, and then were replated in 2 ug/mL puromycin selective media. Cells were collected in TriZol LS reagent for RNA for microarrays ~48 hours after start of puromycin selection.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from Trizol LS according to manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
We used the Ambion WT Labelling Kit as per manufacturer's instructions. We used 250 ng/sample of starting total RNA.
|
|
|
Hybridization protocol |
Biotinylated DNA probe was made according to the standard Affymetrix protocol.
|
Scan protocol |
Arrays were scanned according to the Affymetrix protocol.
|
Data processing |
Gene level RMA
|
|
|
Submission date |
Mar 18, 2013 |
Last update date |
Dec 31, 2014 |
Contact name |
Colin Palmer |
E-mail(s) |
cjp2123@columbia.edu
|
Organization name |
Columbia University
|
Department |
Microbiology & Immunology
|
Lab |
Reizis Lab
|
Street address |
701 W. 168th Street
|
City |
New York |
State/province |
New York |
ZIP/Postal code |
10032 |
Country |
USA |
|
|
Platform ID |
GPL6244 |
Series (2) |
GSE45265 |
Effect of lentiviral-based shRNA-mediated knockdown of ZFX on the human medulloblastoma (MB) cell-line DAOY |
GSE45396 |
Transcription factor ZFX |
|