|
Status |
Public on May 28, 2013 |
Title |
MGG4_rep2 |
Sample type |
genomic |
|
|
Source name |
MGG4 human glioblastoma stem cell line
|
Organism |
Homo sapiens |
Characteristics |
cell type: MGG4 cell line: human glioblastoma stem cells
|
Growth protocol |
MGG cells were cultured and passaged using standard neural stem cell conditions as described Wakimoto et al, 2009 (PMID: 19351838)
|
Extracted molecule |
genomic DNA |
Extraction protocol |
DNA was extracted using the QiaAMP DNA mini kit per the manufacturer's instructions
|
Label |
Cy3 and Cy5
|
Label protocol |
Standard Illumina protocol
|
|
|
Hybridization protocol |
Bisulfite conversion with Zymo EZ DNA methylation kit followed by standard Illumina protocol
|
Scan protocol |
ChIPs were scanned on Illumina iScans
|
Data processing |
Data processing performed with Illumina BeadStudio software (v. 3.1.3.0). The non_normalized.txt contains unmethylated and methylated signal intensities.
|
|
|
Submission date |
Apr 12, 2013 |
Last update date |
May 28, 2013 |
Contact name |
Esther Rheinbay |
E-mail(s) |
esther@broadinstitute.org
|
Organization name |
Broad Institute
|
Street address |
7 Cambridge Center
|
City |
Cambridge |
State/province |
MA |
ZIP/Postal code |
02142 |
Country |
USA |
|
|
Platform ID |
GPL8490 |
Series (2) |
GSE46015 |
DNA methylation profiling of glioblastoma cancer stem cells |
GSE46016 |
Epigenomic profiling of glioblastoma stem cells |
|