|
| Status |
Public on May 28, 2013 |
| Title |
MGG6_rep2 |
| Sample type |
genomic |
| |
|
| Source name |
MGG6 human glioblastoma stem cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: MGG6
cell line: human glioblastoma stem cells
|
| Growth protocol |
MGG cells were cultured and passaged using standard neural stem cell conditions as described Wakimoto et al, 2009 (PMID: 19351838)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted using the QiaAMP DNA mini kit per the manufacturer's instructions
|
| Label |
Cy3 and Cy5
|
| Label protocol |
Standard Illumina protocol
|
| |
|
| Hybridization protocol |
Bisulfite conversion with Zymo EZ DNA methylation kit followed by standard Illumina protocol
|
| Scan protocol |
ChIPs were scanned on Illumina iScans
|
| Data processing |
Data processing performed with Illumina BeadStudio software (v. 3.1.3.0). The non_normalized.txt contains unmethylated and methylated signal intensities.
|
| |
|
| Submission date |
Apr 12, 2013 |
| Last update date |
May 28, 2013 |
| Contact name |
Esther Rheinbay |
| E-mail(s) |
esther@broadinstitute.org
|
| Organization name |
Broad Institute
|
| Street address |
7 Cambridge Center
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platform ID |
GPL8490 |
| Series (2) |
| GSE46015 |
DNA methylation profiling of glioblastoma cancer stem cells |
| GSE46016 |
Epigenomic profiling of glioblastoma stem cells |
|
