|
| Status |
Public on May 02, 2014 |
| Title |
A375 control rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
A375 control
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: A375
cell type: melanoma
|
| Treatment protocol |
Cancer cell lines and lymphocytes were grown in in a CO2 incubator and PST-Dox nanoparticles were administered for 24 hr for the treatement groups and total RNA was isolated using Trizol extration protocols.
|
| Growth protocol |
Cancer cell lines were grown in 10 % DMEM and Lymphocytes in 10 % RPMI in a CO2 incubator.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from the total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized for 16 hr at 45C on GeneChip Human Whole Genome Array.
|
| Scan protocol |
Scanning were performed per recommended specifications
|
| Description |
Gene expression profiling of Human melanoma cancer cell line
|
| Data processing |
Raw data obtained in .cel format were analyzed using GeneSpring GX v 12.0. RMA method was used for probe summarization and normalization was done to median of tissue specific samples.
|
| |
|
| Submission date |
May 02, 2013 |
| Last update date |
May 02, 2014 |
| Contact name |
madavan vasudevan |
| E-mail(s) |
madavan.vasudevan@gmail.com
|
| Phone |
+91-9845093355
|
| Organization name |
Theomics International Private Limited
|
| Street address |
Kasturi Nagar
|
| City |
Bangalore |
| State/province |
Karnataka |
| ZIP/Postal code |
560043 |
| Country |
India |
| |
|
| Platform ID |
GPL15207 |
| Series (1) |
| GSE46570 |
Gene expression analysis of PST-Dox nanoparticles on isolated normal lymphocytes and in cancer cell lines |
|
