|
| Status |
Public on Jul 12, 2013 |
| Title |
Parental Dox Replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
Parental iPS cell line Dox Replicate 3
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: induced Pluripotent Stem cells
cell line type: parental
treatment: Doxycycline
|
| Treatment protocol |
+ or - doxycycline (ug/ml) for 22days
|
| Growth protocol |
DS iPSC parent line (DS1-iPS4) was provided by George Q. Daley (Children’s Hospital Boston, USA) and maintained on irradiated mouse embryonic fibroblasts (iMEFs) (R & D Systems, PSC001) in hiPSC medium containing DNEM/F12 supplemented with 20% knockout Serum Replacement (Invitrogen), 1mM glutamine (Invitrogen), 100 µM non-essential amino acids (Invitrogen), 100 µM β-mercaptoethanol (Sigma) and 10 ng/ml FGF-β (Invitrogen, PHG0024). Cultures were passaged every 5-7 days with 1 mg/ml of collagenase type IV (Invitrogen).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
High Pure RNA extraction kit (Roche) was used to extract RNA and was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Gene Chip 3' IVT Express Kit was used to label RNA from 1 ug total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Primeview Human Gene Expression Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 with 7g upgrade.
|
| Description |
Gene expression data from iPS cell lines treated with Doxycycline
JJ12
|
| Data processing |
The data were analyzed with Affymetrix Gene Expression Console. RMA normalization was applied to all samples.
|
| |
|
| Submission date |
May 16, 2013 |
| Last update date |
Jul 12, 2013 |
| Contact name |
Benjamin R Carone |
| E-mail(s) |
ben.carone@gmail.com
|
| Organization name |
UMass Medical School
|
| Department |
BMP
|
| Street address |
364 Plantation St
|
| City |
Worcester |
| State/province |
Massachusetts |
| ZIP/Postal code |
01605 |
| Country |
USA |
| |
|
| Platform ID |
GPL15207 |
| Series (1) |
| GSE47014 |
Translating Dosage Compensation to Trisomy 21 |
|
