|
Status |
Public on Dec 31, 2013 |
Title |
KBM7 bio rep1 tech rep2 |
Sample type |
SRA |
|
|
Source name |
KBM7
|
Organism |
Homo sapiens |
Characteristics |
ploidy: haploid cell line: KBM7
|
Treatment protocol |
none
|
Growth protocol |
KBM7 cell line was cultured according to standard cell culture procedures
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using TRIZol (Sigma) and ribosomal RNAs were depleted using RiboZero (Epicentre). Libraries were constructed using the ScriptSeq v1 (Epicentre) KIT.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2000 |
|
|
Description |
technical replicate 2 strand specific RNA-Seq using tagged hexamers
|
Data processing |
basecalling, alignment of both technical replicates (TopHat v2.0.3 using Bowtie 2.0.0.6), genome build: hg19, processed data files format and content: tab delimited text file of RPKM counts (KBM7_RefSeq_RPKM)
|
|
|
Submission date |
May 28, 2013 |
Last update date |
May 15, 2019 |
Contact name |
Florian M Pauler |
E-mail(s) |
florian.pauler@ist.ac.at
|
Phone |
+43 2243 9000-7434
|
Organization name |
IST Austria
|
Lab |
Simon Hippenmeyer
|
Street address |
Am Campus 1
|
City |
Klosterneuburg |
ZIP/Postal code |
3400 |
Country |
Austria |
|
|
Platform ID |
GPL11154 |
Series (1) |
GSE47428 |
A haploid gene-trap mutant collection enables functional genetics in human cells |
|
Relations |
BioSample |
SAMN02179866 |
SRA |
SRX286828 |