|
| Status |
Public on Feb 13, 2014 |
| Title |
MCF7_Hypoxia_48h_rep3 [mRNA] |
| Sample type |
RNA |
| |
|
| Source name |
MCF7_Hypoxia_48h
|
| Organism |
Homo sapiens |
| Characteristics |
oxygen: 1%
time of exposure: 48h
|
| Treatment protocol |
Exposure of cell cultures to hypoxia (1% oxygen) was undertaken in an In vivo2 Hypoxia Work Station (Ruskin Technologies) in parallel with cells maintained in normoxic conditions (21% oxygen). Cells were recovered after 16h, 32h and 48h of exposure to hypoxia.
|
| Growth protocol |
MCF-7 cells were grown in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum and 2mmol/L of L-glutamine.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from cells using the miRVana miRNA isolation kit (Ambion) in accordance with the prescribed protocol provided with the kit. RNA quality was assessed after extraction using Agilent bioanalyser.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion TotalPrep RNA Amplification kit.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Hypoxia_48h_R3
replicate 3
|
| Data processing |
The data were log2 transformed and normalised (quantile normalisation) using preprocessCore package from Bioconductor
|
| |
|
| Submission date |
May 30, 2013 |
| Last update date |
Feb 13, 2014 |
| Contact name |
Carme Camps |
| Organization name |
Wellcome Trust Centre for Human Genetics/ U. of Oxford
|
| Street address |
Roosevelt Drive
|
| City |
Oxford |
| ZIP/Postal code |
OX37BN |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6884 |
| Series (2) |
| GSE47533 |
Integrated analysis of microRNA and mRNA expression and association with HIF binding in MCF-7 cells under hypoxia [mRNA] |
| GSE47534 |
Integrated analysis of microRNA and mRNA expression and association with HIF binding in MCF-7 cells under hypoxia |
|
