|
| Status |
Public on Jun 11, 2014 |
| Title |
AhR KO untreated 2 |
| Sample type |
RNA |
| |
|
| Source name |
AhR KO untreated
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: AhR-/-
tissue type: skin
|
| Treatment protocol |
Skin samples were snap-frozen immediately after collection.
|
| Growth protocol |
Skin samples were collected at day 0 or day 2 following initiation of imiquimod treatment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from mouse skin was obtained using a Polytron PT 3000 tissue homogenizer (Kinematika) along with TRIzol (Life technologies), according to the manufacturers’ instructions.
|
| Label |
biotin
|
| Label protocol |
User guide Affymetrix GeneChip 3’ Express Kit (P/N 702646 Rev.8)
|
| |
|
| Hybridization protocol |
User guide Affymetrix GeneChip 3’ Express Kit (P/N 702646 Rev.8)
|
| Scan protocol |
User guide Affymetrix Expression Wash, Stain and Scan (P/N 702731 Rev. 3)
|
| Description |
biological replicate 2
|
| Data processing |
The data were RMA normalized using R package affy (Bolstad et al., 2003) that is a part of the Bioconductor project http://www.bioconductor.org.
|
| |
|
| Submission date |
Jun 03, 2013 |
| Last update date |
Jun 12, 2014 |
| Contact name |
Gitta Stockinger |
| E-mail(s) |
bstocki@nimr.mrc.ac.uk
|
| Organization name |
National Institute for Medical Research
|
| Department |
Division of Molecular Immunology
|
| Street address |
The Ridgeway
|
| City |
London |
| ZIP/Postal code |
NW7 1AA |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE47607 |
Environmental factors transmitted by aryl hydrocarbon receptor influence severity of psoriatic skin inflammation [Affymetrix] |
| GSE47965 |
Environmental factors transmitted by aryl hydrocarbon receptor influence severity of psoriatic skin inflammation |
|
