|
Status |
Public on Jun 11, 2014 |
Title |
AhR KO 2d Imiquimod 3 |
Sample type |
RNA |
|
|
Source name |
AhR KO 2d Imiquimod
|
Organism |
Mus musculus |
Characteristics |
genotype/variation: AhR-/- tissue type: skin
|
Treatment protocol |
Skin samples were snap-frozen immediately after collection.
|
Growth protocol |
Skin samples were collected at day 0 or day 2 following initiation of imiquimod treatment.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA from mouse skin was obtained using a Polytron PT 3000 tissue homogenizer (Kinematika) along with TRIzol (Life technologies), according to the manufacturers’ instructions.
|
Label |
biotin
|
Label protocol |
User guide Affymetrix GeneChip 3’ Express Kit (P/N 702646 Rev.8)
|
|
|
Hybridization protocol |
User guide Affymetrix GeneChip 3’ Express Kit (P/N 702646 Rev.8)
|
Scan protocol |
User guide Affymetrix Expression Wash, Stain and Scan (P/N 702731 Rev. 3)
|
Description |
biological replicate 3
|
Data processing |
The data were RMA normalized using R package affy (Bolstad et al., 2003) that is a part of the Bioconductor project http://www.bioconductor.org.
|
|
|
Submission date |
Jun 03, 2013 |
Last update date |
Jun 12, 2014 |
Contact name |
Gitta Stockinger |
E-mail(s) |
bstocki@nimr.mrc.ac.uk
|
Organization name |
National Institute for Medical Research
|
Department |
Division of Molecular Immunology
|
Street address |
The Ridgeway
|
City |
London |
ZIP/Postal code |
NW7 1AA |
Country |
United Kingdom |
|
|
Platform ID |
GPL1261 |
Series (2) |
GSE47607 |
Environmental factors transmitted by aryl hydrocarbon receptor influence severity of psoriatic skin inflammation [Affymetrix] |
GSE47965 |
Environmental factors transmitted by aryl hydrocarbon receptor influence severity of psoriatic skin inflammation |
|