|
| Status |
Public on Jun 05, 2013 |
| Title |
KYSE180 cells transfected with siRNAs targeting HOTAIR |
| Sample type |
RNA |
| |
|
| Source name |
HOTAIR cell line transfected with HOTAIR siRNAs usng Lipofectamine2000
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: esophageal squanmous cell carcinoma (ESCC) cells
cell line: KYSE180
treatment: siRNAs targeting HOTAIR
|
| Treatment protocol |
KYSE180 cells were transfected with HOTAIR vector and HOTAIR siRNAs, respectively for 48 hours.
|
| Growth protocol |
KYSE180 cells were maintained in RMPI1640 supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using TRIZOL Reagent following the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotin: GeneChip ® 3' IVT Express labeling Kit
|
| |
|
| Hybridization protocol |
FS450_0002 for the human GeneChip PrimeView arrays
|
| Scan protocol |
Affymetrix GeneChip Scanner 7G
|
| Description |
gene expression data from HOTAIR cell line transfected with HOTAIR siRNAs usng Lipofectamine2000
|
| Data processing |
GeneChip Command Console AGCC software 3.2.4 was used to generate the CEL files. The computational pipeline was run on the bioinformatics platform GenomeSpac. Data were extracted from the CEL files and preprocessed by applying RMA and the quantile normalization procedures implemented in the ExpressionFileCreator module of the GenePattern software platform.
|
| |
|
| Submission date |
Jun 04, 2013 |
| Last update date |
Jun 05, 2013 |
| Contact name |
Weidong Han |
| E-mail(s) |
hanwdrsw69@yahoo.com
|
| Organization name |
Chinese PLA General Hospital
|
| Department |
School of Life Sciences
|
| Lab |
Department of Molecular Biology
|
| Street address |
#28 Fuxing Road, HaiDian District
|
| City |
Beijing |
| ZIP/Postal code |
100853 |
| Country |
China |
| |
|
| Platform ID |
GPL15207 |
| Series (2) |
| GSE47638 |
HOTAIR reprograms gene expression profiling in esophageal squamous cell carcinoma (ESCC) KYSE180 cells |
| GSE47639 |
KYSE180 cells transfected with HOTAIR compared to control cells |
|
