|
| Status |
Public on Jun 22, 2016 |
| Title |
Culture neuron -RNAi electroporation 48h |
| Sample type |
SRA |
| |
|
| Source name |
neurons electroporated with RNAi plasmid
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague-Dawley
Stage: E16
tissue: brain
|
| Treatment protocol |
Dissociated cells were electroporated with control plasmid or RNAi plasmid
|
| Growth protocol |
Somatosensory cortexs of E16 rats' brain were dissected and dissociated into single cells. Medium contained Neurobasal medium, 10% FBS and 2% B27. Neurons were cultured for 48 hours
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Base-calling performed by using Illumina software
Removing 3’ adaptor sequence, empty reads, low quality Tags, Tags with a copy number of 1 and those Tags are too long or too short.
Sequenced reads were mapped to rn4 whole genome
Genome_build: rn4
Supplementary_files_format_and_content: text file containing gene expression
|
| |
|
| Submission date |
Jun 24, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Pingping Zhao |
| E-mail(s) |
ppzhao@ion.ac.cn
|
| Phone |
8613671699349
|
| Organization name |
Institute of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
|
| Street address |
No. 320, YueYang Road, Shanghai, China
|
| City |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
| |
|
| Platform ID |
GPL14844 |
| Series (1) |
| GSE48236 |
Genome-wide analysis of rats' mRNA profile after knocking down PIWIL1 in embryonic cultured neurons. |
|
| Relations |
| BioSample |
SAMN02212504 |
| SRA |
SRX314621 |
