|
| Status |
Public on Nov 05, 2013 |
| Title |
GR3KR_dex_6h_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Isogenic HEK293 Cells, dex treated, 6 h
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Isogenic Human Embryonal Kidney (HEK293) Cells
treatment: 100 nM of dexamethasone
time: 6 h
|
| Treatment protocol |
3x105x of Isogenic HEK293 cells were seeded at 6-well plates and allowed to grow in steroid-depleted medium (2.5 % charcoal stripped FBS in DMEM) 36 h and the cells were subsequently treated with 100 nM of dexamethasone for 6 h prior extraction of RNA
|
| Growth protocol |
Isogenic HEK293 cells were grown in Dulbecco's Modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum, 25 U/ml penicillin and 25 µg/ml streptomycin, and 100 µg hygromycin-B, and kept at 37 °C in a humidified 95% air/ 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using TriPure (Roche Diagnostics GmbH, Mannheim, Germany) according to manufacture's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmk kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standart Illumina hybridization protocol
|
| Scan protocol |
Standart Illumina scanning protocol
|
| Description |
stably expressing GR3KR
3KR_10
|
| Data processing |
Obtained data was normalized using VST transformation and RSN normalization used as standard approach for Illumina arrays with the R/lumi package
|
| |
|
| Submission date |
Jun 26, 2013 |
| Last update date |
Nov 05, 2013 |
| Contact name |
Ville Paakinaho |
| E-mail(s) |
villepaakinaho@gmail.com
|
| Organization name |
University of Eastern Finland
|
| Department |
School of Medicine
|
| Lab |
Biomedicine
|
| Street address |
Yliopistonranta 8
|
| City |
Kuopio |
| ZIP/Postal code |
70210 |
| Country |
Finland |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE48328 |
SUMOylation Regulates the Anti-Proliferative Gene Signature Programs of Glucocorticoid Receptor (HEK293) |
| GSE48379 |
SUMOylation Regulates the Anti-Proliferative Gene Signature Programs of Glucocorticoid Receptor |
|
