|
Status |
Public on Nov 05, 2013 |
Title |
U2Os GR3KR_dex_6h_rep1 |
Sample type |
RNA |
|
|
Source name |
U2Os Cells, dex treated, 6 h
|
Organism |
Homo sapiens |
Characteristics |
cell line: U2 Osteosarcoma (U2Os) Cells treatment: 100 nM of dexamethasone time: 6 h
|
Treatment protocol |
2.5x105x of U2Os cells were seeded at 6-well plates and allowed to grow in steroid-depleted medium (2.5 % charcoal stripped FBS in DMEM) 36 h and the cells were subsequently treated with 100 nM of dexamethasone for 6 h prior extraction of RNA
|
Growth protocol |
U2Os cells were grown in Dulbecco's Modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum, 1 mM Na-puryvate, 25 U/ml penicillin and 25 µg/ml streptomycin, and 400 µg geneticin, and kept at 37 °C in a humidified 95% air/ 5% CO2
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using TriPure (Roche Diagnostics GmbH, Mannheim, Germany) according to manufacture's instructions
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmk kit for Illumina arrays
|
|
|
Hybridization protocol |
Standart Illumina hybridization protocol
|
Scan protocol |
Standart Illumina scanning protocol
|
Description |
stably expressing GR3KR
|
Data processing |
Obtained data was normalized using VST transformation and RSN normalization used as standard approach for Illumina arrays with the R/lumi package
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|
|
Submission date |
Jun 26, 2013 |
Last update date |
Nov 05, 2013 |
Contact name |
Ville Paakinaho |
E-mail(s) |
villepaakinaho@gmail.com
|
Organization name |
University of Eastern Finland
|
Department |
School of Medicine
|
Lab |
Biomedicine
|
Street address |
Yliopistonranta 8
|
City |
Kuopio |
ZIP/Postal code |
70210 |
Country |
Finland |
|
|
Platform ID |
GPL10558 |
Series (2) |
GSE48330 |
SUMOylation Regulates the Anti-Proliferative Gene Signature Programs of Glucocorticoid Receptor (U2Os cell line) |
GSE48379 |
SUMOylation Regulates the Anti-Proliferative Gene Signature Programs of Glucocorticoid Receptor |
|