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Sample GSM1179392 Query DataSets for GSM1179392
Status Public on Aug 30, 2013
Title 30nM_Topotecan
Sample type RNA
 
Source name cultured cortical neurons
Organism Mus musculus
Characteristics treatment: 30nM_Topotecan
cell type: cultured cortical neurons
strain: C57BL6
Treatment protocol After 7 days in vitro, mouse cortical neurons were treated with drug or vehicle. After 24 or 72 hrs drug treatement, total RNA was prepared.
Growth protocol Mouse cortical neurons were cultured from E13-15 mouse embryos for 7 days in vitro.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from mouse cortical neurons using Trizol reagent.
Label biotin
Label protocol RNA was labeled using the Affymetrix/Ambion WT Expression kit, combined with Affymetrix GeneChip WT terminal labeling kit.
 
Hybridization protocol Arrays for experiments (1) and (3) were washed and stained using the Affymetrix Gene Fluidics station 450. Hybridization was performed at 48˚C for 17 hours. Arrays for other experiments were run using the Affymetrix GeneTitan system. Steps subsequent to hybridization were performed with the Beckman Coulter FXP Target Express Robot. Hybridization was performed at 45˚C for 16 hours with the Affymetrix GeneTitan instrument, controlled using AGCC software.
Scan protocol GeneTitan arrays were scanned using the Affymetrix GeneTitan instrument, controlled by AGCC software. Individual arrays were scanned using the Affymetrix GeneChip Scanner 3000 7G Plus with autoloader.
Description MoGene-1_1-st-v1
Gene Expression data for Topotecan treated mouse cultured cortical neurons
Data processing Arrays were normalized and expression values determined using Affymetrix Expression Console. Linear RMA normalization was used for all experiments.
 
Submission date Jul 02, 2013
Last update date Aug 30, 2013
Contact name Chandri Yandava
E-mail(s) yandava@email.unc.edu
Organization name University of North Carolina at Chapel Hill
Department Carolina Institute for Developmental Disabilities
Street address 101 Renee Lynne Court
City Carrboro
State/province NC
ZIP/Postal code 27510
Country USA
 
Platform ID GPL11533
Series (2)
GSE43899 Co-ordinate inhibiton of autism candidate genes by topoisomerase inhibitors [array]
GSE43900 Co-ordinate inhibition of autism candidate genes by topoisomerase inhibitors

Data table header descriptions
ID_REF
VALUE Linear RMA signal

Data table
ID_REF VALUE
10344614 119.25
10344616 6.572338
10344620 10.48983
10344622 38.13561
10344624 544.3873
10344633 572.8952
10344637 1535.008
10344653 12.25234
10344658 456.8821
10344674 11.13983
10344679 46.87358
10344707 900.7919
10344713 603.629
10344715 17.5772
10344717 9.630413
10344719 23.4821
10344721 6.159724
10344723 115.421
10344725 150.365
10344741 1164.513

Total number of rows: 35556

Table truncated, full table size 621 Kbytes.




Supplementary file Size Download File type/resource
GSM1179392_30nM_Topotecan.CEL.gz 4.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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