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Sample GSM1179413 Query DataSets for GSM1179413
Status Public on Aug 30, 2013
Title Topotecan_washout_And_Irinotecan_10µM_vehicle_2
Sample type RNA
 
Source name cultured cortical neurons
Organism Mus musculus
Characteristics treatment: Topotecan_washout_And_Irinotecan_10µM_vehicle
cell type: cultured cortical neurons
strain: C57BL6
Treatment protocol After 7 days in vitro, mouse cortical neurons were treated with drug or vehicle. After 24 or 72 hrs drug treatement, total RNA was prepared.
Growth protocol Mouse cortical neurons were cultured from E13-15 mouse embryos for 7 days in vitro.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from mouse cortical neurons using Trizol reagent.
Label biotin
Label protocol RNA was labeled using the Affymetrix/Ambion WT Expression kit, combined with Affymetrix GeneChip WT terminal labeling kit.
 
Hybridization protocol Arrays for experiments (1) and (3) were washed and stained using the Affymetrix Gene Fluidics station 450. Hybridization was performed at 48˚C for 17 hours. Arrays for other experiments were run using the Affymetrix GeneTitan system. Steps subsequent to hybridization were performed with the Beckman Coulter FXP Target Express Robot. Hybridization was performed at 45˚C for 16 hours with the Affymetrix GeneTitan instrument, controlled using AGCC software.
Scan protocol GeneTitan arrays were scanned using the Affymetrix GeneTitan instrument, controlled by AGCC software. Individual arrays were scanned using the Affymetrix GeneChip Scanner 3000 7G Plus with autoloader.
Description MoGene-2_1-st
Gene Expression data for dose response to Topotecan treatment of mouse cultured cortical neurons
Data processing Arrays were normalized and expression values determined using Affymetrix Expression Console. Linear RMA normalization was used for all experiments.
 
Submission date Jul 02, 2013
Last update date Aug 30, 2013
Contact name Chandri Yandava
E-mail(s) yandava@email.unc.edu
Organization name University of North Carolina at Chapel Hill
Department Carolina Institute for Developmental Disabilities
Street address 101 Renee Lynne Court
City Carrboro
State/province NC
ZIP/Postal code 27510
Country USA
 
Platform ID GPL17400
Series (2)
GSE43899 Co-ordinate inhibiton of autism candidate genes by topoisomerase inhibitors [array]
GSE43900 Co-ordinate inhibition of autism candidate genes by topoisomerase inhibitors

Data table header descriptions
ID_REF
VALUE Linear RMA signal

Data table
ID_REF VALUE
17210850 3.596763
17210852 4.434215
17210855 184.677
17210869 354.3643
17210883 9.843461
17210887 525.1792
17210904 21.9921
17210912 501.4305
17210947 12.33109
17210953 51.08536
17210984 426.8905
17210994 16.01174
17210996 4.17557
17210998 4.980103
17211000 66.15595
17211004 114.5136
17211023 395.217
17211033 12.48448
17211043 97.80846
17211066 22.42037

Total number of rows: 41345

Table truncated, full table size 722 Kbytes.




Supplementary file Size Download File type/resource
GSM1179413_vehicle_For_Topotecan_washout_And_Irinotecan_10uM_2.CEL.gz 4.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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