|
| Status |
Public on Aug 30, 2013 |
| Title |
Topotecan_1µM_vehicle_2 |
| Sample type |
RNA |
| |
|
| Source name |
cultured cortical neurons
|
| Organism |
Mus musculus |
| Characteristics |
treatment: Topotecan_1µM_vehicle
cell type: cultured cortical neurons
strain: C57BL6
|
| Treatment protocol |
After 7 days in vitro, mouse cortical neurons were treated with drug or vehicle. After 24 or 72 hrs drug treatement, total RNA was prepared.
|
| Growth protocol |
Mouse cortical neurons were cultured from E13-15 mouse embryos for 7 days in vitro.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from mouse cortical neurons using Trizol reagent.
|
| Label |
biotin
|
| Label protocol |
RNA was labeled using the Affymetrix/Ambion WT Expression kit, combined with Affymetrix GeneChip WT terminal labeling kit.
|
| |
|
| Hybridization protocol |
Arrays for experiments (1) and (3) were washed and stained using the Affymetrix Gene Fluidics station 450. Hybridization was performed at 48˚C for 17 hours. Arrays for other experiments were run using the Affymetrix GeneTitan system. Steps subsequent to hybridization were performed with the Beckman Coulter FXP Target Express Robot. Hybridization was performed at 45˚C for 16 hours with the Affymetrix GeneTitan instrument, controlled using AGCC software.
|
| Scan protocol |
GeneTitan arrays were scanned using the Affymetrix GeneTitan instrument, controlled by AGCC software. Individual arrays were scanned using the Affymetrix GeneChip Scanner 3000 7G Plus with autoloader.
|
| Description |
MoGene-2_1-st
Gene Expression data for dose response to Topotecan treatment of mouse cultured cortical neurons
|
| Data processing |
Arrays were normalized and expression values determined using Affymetrix Expression Console. Linear RMA normalization was used for all experiments.
|
| |
|
| Submission date |
Jul 02, 2013 |
| Last update date |
Aug 30, 2013 |
| Contact name |
Chandri Yandava |
| E-mail(s) |
yandava@email.unc.edu
|
| Organization name |
University of North Carolina at Chapel Hill
|
| Department |
Carolina Institute for Developmental Disabilities
|
| Street address |
101 Renee Lynne Court
|
| City |
Carrboro |
| State/province |
NC |
| ZIP/Postal code |
27510 |
| Country |
USA |
| |
|
| Platform ID |
GPL17400 |
| Series (2) |
| GSE43899 |
Co-ordinate inhibiton of autism candidate genes by topoisomerase inhibitors [array] |
| GSE43900 |
Co-ordinate inhibition of autism candidate genes by topoisomerase inhibitors |
|
