|
| Status |
Public on Jul 04, 2013 |
| Title |
Bone marrow 100 clone LOW |
| Sample type |
RNA |
| |
|
| Source name |
MSC clone after 28 days of culture, CD146Low
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: cultivated bone marrow-derived mesenchymal stem cells
surface cd146: low
donor: 100
|
| Treatment protocol |
No treatment.
|
| Growth protocol |
MSC non-clonal or clonal were cultivated with aMEM (minimum essential medium) + 10% FCS (fetal calf serum) + 10µg/ml ciprofloxacin during 21 and 28 days, respectively.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Use of RNeasy kit (Qiagen). RNA purity and integrity were checked by use of Bioanalyzer 2100 (Agilent).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was amplified by the small sample labelling protocol (two cycle amplification kit, Affymetrix).
|
| |
|
| Hybridization protocol |
Hybridized on GeneChip HuGene 1.0 ST oligonucleotide microarrays (Affymetrix).
|
| Scan protocol |
GeneChip scanner 3000 and software AGCC.
|
| Description |
MoPur 100 LOW
Gene expression data from human cultivated bone marrow-derived MSC during 28 days.
|
| Data processing |
Expression signal values and P-values were obtained for each probe set by use of Partek Genomics suite (Partek) by the Robust Multichip Averaging algorithm in normalization. Principal component and gene expression analysis involved use of MeV.
|
| |
|
| Submission date |
Jul 03, 2013 |
| Last update date |
Jul 04, 2013 |
| Contact name |
Nicolas Espagnolle |
| E-mail(s) |
nicolas.espagnolle@efs.sante.fr
|
| Organization name |
STROMALab, Université de Toulouse, CNRS ERL5311, EFS, INP-ENVT, Inserm U1031, UPS, Toulouse, France
|
| Street address |
Bâtiment INCERE 4 bis, avenue Hubert Curien
|
| City |
Toulouse |
| ZIP/Postal code |
31100 |
| Country |
France |
| |
|
| Platform ID |
GPL10739 |
| Series (1) |
| GSE48540 |
CD146 expression in mesenchymal stem cells is associated with vascular smooth muscle commitment |
|
