|
| Status |
Public on Nov 06, 2013 |
| Title |
ED32 |
| Sample type |
SRA |
| |
|
| Source name |
cord blood cells
|
| Organism |
Homo sapiens |
| Characteristics |
developmental stage: peri-natal
tissue: umbilical cord blood
sorted cell type: CD34+CD45RA-
|
| Treatment protocol |
Sorting by FACS of CD34+ and CD34+CD45RA- cells.
|
| Growth protocol |
Primary cells.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TriZol + RNeasy mini Kit (Qiagen, #74106).
TruSeq RNA Sample Preparation (Illumina).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Basecalls performed using CASAVA 1.8.2.
Sequenced reads were trimmed for adaptor sequence and mapped to the reference genome (hg19) using CASAVA 1.8.2.
Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated using CASAVA 1.8.2.
Genome_build: GRCh37
Supplementary_files_format_and_content: Tab-delimited text files include RPKM values for each sample. Columns are: Chromosome, Start location, Stop location, Gene, RPKM, base count.
|
| |
|
| Submission date |
Jul 12, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Guy Sauvageau |
| Organization name |
Institute for Research in Immunology and Cancer
|
| Street address |
2950, Chemin de Polytechnique
|
| City |
Montreal |
| State/province |
QC |
| ZIP/Postal code |
H3T 1J4 |
| Country |
Canada |
| |
|
| Platform ID |
GPL11154 |
| Series (2) |
| GSE48173 |
Leucegene |
| GSE48846 |
Transcriptome of Primitive Human Hematopoietic Cells: A New Resource to Find hHSC-Specific Genes |
|
| Relations |
| BioSample |
SAMN02232140 |
| SRA |
SRX322294 |
