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Sample GSM1185618 Query DataSets for GSM1185618
Status Public on Nov 06, 2013
Title ED38
Sample type SRA
 
Source name cord blood cells
Organism Homo sapiens
Characteristics developmental stage: peri-natal
tissue: umbilical cord blood
sorted cell type: CD34+CD45RA-
Treatment protocol Sorting by FACS of CD34+ and CD34+CD45RA- cells.
Growth protocol Primary cells.
Extracted molecule total RNA
Extraction protocol TriZol + RNeasy mini Kit (Qiagen, #74106).
TruSeq RNA Sample Preparation (Illumina).
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
 
Data processing Basecalls performed using CASAVA 1.8.2.
Sequenced reads were trimmed for adaptor sequence and mapped to the reference genome (hg19) using CASAVA 1.8.2.
Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated using CASAVA 1.8.2.
Genome_build: GRCh37
Supplementary_files_format_and_content: Tab-delimited text files include RPKM values for each sample. Columns are: Chromosome, Start location, Stop location, Gene, RPKM, base count.
 
Submission date Jul 12, 2013
Last update date May 15, 2019
Contact name Guy Sauvageau
Organization name Institute for Research in Immunology and Cancer
Street address 2950, Chemin de Polytechnique
City Montreal
State/province QC
ZIP/Postal code H3T 1J4
Country Canada
 
Platform ID GPL11154
Series (2)
GSE48173 Leucegene
GSE48846 Transcriptome of Primitive Human Hematopoietic Cells: A New Resource to Find hHSC-Specific Genes
Relations
BioSample SAMN02232139
SRA SRX322297

Supplementary file Size Download File type/resource
GSM1185618_ED38_RPKM.txt.gz 420.8 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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