|
| Status |
Public on Dec 17, 2013 |
| Title |
Nuclear_Donor |
| Sample type |
genomic |
| |
|
| Source name |
Nuclear_Donor blood
|
| Organism |
Canis lupus familiaris |
| Characteristics |
breed: Labrador Retriever
gender: Male
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
genomic DNA extracted using DNeasy Blood & Tissue Kit (Qiagen) following manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
200ng of genomic DNA was whole-genome amplified in an overnight reaction. After incubation the amplified DNA was fragmented with fragmentation mix, precipitated with isopropanol and precipitation mix and resuspended in hybridization buffer.
|
| |
|
| Hybridization protocol |
Each fragmented DNA was purified and mixed with hybridization reagents, applied on the BeadChip and incubated for 16 hours. After hybridization, single-base extension and allele-specific staining was performed on a Teflow chamber rack system sequentially.
|
| Scan protocol |
The BeadChip were scanned using Illumina BeadStation 500G array scanner
|
| Description |
Nuclear_Donor
|
| Data processing |
Intensity files were processed by GenomeStudio V2011.1, GT module 1.9.4 with default analysis settings. Each SNP is analyzed independently to cluster and identify genotypes. Genotype calls are generated by comparing experimental data with those in the supplied cluster file.
|
| |
|
| Submission date |
Jul 23, 2013 |
| Last update date |
Dec 17, 2013 |
| Contact name |
SeungHyun Jung |
| E-mail(s) |
hyun@catholic.ac.kr
|
| Phone |
82-2-2258-7509
|
| Organization name |
Catholic University of Korea
|
| Department |
Department of Biochemistry
|
| Street address |
catholic university, medical college, banpo-dong
|
| City |
seoul |
| State/province |
seoul |
| ZIP/Postal code |
06591 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL17481 |
| Series (2) |
| GSE49123 |
De novo copy number variations in cloned dogs (Illumina SNP) |
| GSE49357 |
De novo copy number variations in cloned dogs |
|
