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Sample GSM1196523 Query DataSets for GSM1196523
Status Public on Apr 29, 2014
Title ACC15
Sample type RNA
 
Source name Frozen tissue of Adrenocortical carcinomas
Organism Homo sapiens
Characteristics age (years): 53
gender: F
cell type: Adrenocortical carcinoma
Growth protocol Samples stored at -80 C
Extracted molecule total RNA
Extraction protocol Tumor samples (10 to 50 mg) were powdered under liquid nitrogen. RNA were extracted using RNAble (Eurobio, Courtaboeuf, France), followed by a clean-up step on RNAeasy columns (Qiagen, Courtaboeuf, France).
Label biotin
Label protocol The labeling of the extracted RNA was performed according to the manufacturer's instruction (Affymetrix)
 
Hybridization protocol Samples were hybridized with GeneChip Human Exon 2.0 ST Arrays (Affymetrix)
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Data processing Raw data were processed with the RMA normalisation (Bioconductor R affy package) + ComBat (R sva package ) to generate log2 normalized intensity signal. Data analysis and statistical evaluations were performed with customized R codes.
 
Submission date Jul 26, 2013
Last update date Apr 29, 2014
Contact name Nabila Elarouci
E-mail(s) Nabila.Elarouci@ligue-cancer.net
Organization name Ligue Nationale contre le Cancer
Department Recherche
Lab Carte d'identité des tumeurs
Street address 14 rue Corvisart
City Paris
ZIP/Postal code 75 013
Country France
 
Platform ID GPL16686
Series (2)
GSE49278 Expression profiling by array of 44 adrenocortical carcinomas
GSE49280 Integrated genomic analyses of adrenocortical tumors (SNP array, DNA methylation, mRNA and miRNA expression).

Data table header descriptions
ID_REF
VALUE Log2 RMA signal

Data table
ID_REF VALUE
16650001 2.915412977
16650003 2.002676118
16650005 3.147336807
16650007 1.442004669
16650009 1.131074879
16650011 3.01769794
16650013 4.681335731
16650015 3.088737588
16650017 1.647577574
16650019 1.959014362
16650021 3.225621345
16650023 1.695496987
16650025 1.595458373
16650027 1.303747618
16650029 3.455739842
16650031 3.475401223
16650033 2.571920624
16650035 1.575376331
16650037 1.29820021
16650041 3.357987378

Total number of rows: 53617

Table truncated, full table size 1093 Kbytes.




Supplementary file Size Download File type/resource
GSM1196523_ACC15.CEL.gz 7.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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