|
| Status |
Public on Apr 29, 2014 |
| Title |
ACC27 |
| Sample type |
RNA |
| |
|
| Source name |
Frozen tissue of Adrenocortical carcinomas
|
| Organism |
Homo sapiens |
| Characteristics |
age (years): 49
gender: F
cell type: Adrenocortical carcinoma
|
| Growth protocol |
Samples stored at -80 C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tumor samples (10 to 50 mg) were powdered under liquid nitrogen. RNA were extracted using RNAble (Eurobio, Courtaboeuf, France), followed by a clean-up step on RNAeasy columns (Qiagen, Courtaboeuf, France).
|
| Label |
biotin
|
| Label protocol |
The labeling of the extracted RNA was performed according to the manufacturer's instruction (Affymetrix)
|
| |
|
| Hybridization protocol |
Samples were hybridized with GeneChip Human Exon 2.0 ST Arrays (Affymetrix)
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
|
| Data processing |
Raw data were processed with the RMA normalisation (Bioconductor R affy package) + ComBat (R sva package ) to generate log2 normalized intensity signal. Data analysis and statistical evaluations were performed with customized R codes.
|
| |
|
| Submission date |
Jul 26, 2013 |
| Last update date |
Apr 29, 2014 |
| Contact name |
Nabila Elarouci |
| E-mail(s) |
Nabila.Elarouci@ligue-cancer.net
|
| Organization name |
Ligue Nationale contre le Cancer
|
| Department |
Recherche
|
| Lab |
Carte d'identité des tumeurs
|
| Street address |
14 rue Corvisart
|
| City |
Paris |
| ZIP/Postal code |
75 013 |
| Country |
France |
| |
|
| Platform ID |
GPL16686 |
| Series (2) |
| GSE49278 |
Expression profiling by array of 44 adrenocortical carcinomas |
| GSE49280 |
Integrated genomic analyses of adrenocortical tumors (SNP array, DNA methylation, mRNA and miRNA expression). |
|
