|
| Status |
Public on Jan 20, 2014 |
| Title |
12 hr Infected Rep2 |
| Sample type |
RNA |
| |
|
| Source name |
HEK293T cells infected 12 hr
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HEK293T
infection: Chikungunya virus
time: 12 h
|
| Treatment protocol |
Infected with an MOI of 1 of chikungunya virus
|
| Growth protocol |
HEK293T cells grown in 6 well plates
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA prepared using Trizol according to manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human PrimeView Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the affymetirx 3000 7G scanner.
|
| Description |
Gene expression data
|
| Data processing |
The raw data were analyzed with Affymetirx Expression Console and Gene springGX 12.0 using Affymetrix default analysis settings and RMA as normalization method used. The probes of intensities less than the 20.0 percentile in the raw data were excluded.
|
| |
|
| Submission date |
Aug 19, 2013 |
| Last update date |
Jan 20, 2014 |
| Contact name |
Dr. Ritu Kulshreshtha |
| E-mail(s) |
ritu@dbeb.iitd.ac.in
|
| Phone |
011-26591017
|
| Organization name |
Indian Institute of Technology
|
| Department |
Dept of Biochemical Engineering and Biotechnology
|
| Lab |
Biochemical Engineering and Biotechnolog
|
| Street address |
Room 134, Block I
|
| City |
New Delhi |
| State/province |
New Delhi |
| ZIP/Postal code |
110016 |
| Country |
India |
| |
|
| Platform ID |
GPL15207 |
| Series (1) |
| GSE49985 |
Gene expression profile in HEK293T cells infected with Chikungunya virus |
|
