|
| Status |
Public on Aug 23, 2014 |
| Title |
AR112Q-KRKR + ligand 2 |
| Sample type |
RNA |
| |
|
| Source name |
PC12 cells expressing AR112Q-KRKR in the presence of androgen
|
| Organism |
Rattus norvegicus |
| Characteristics |
cell line: pheochromocytoma (PC12)
genotype/variation: AR112Q-KRKR
agent: synthetic androgen R1881
|
| Treatment protocol |
PC12 cells were induced to express each AR variant with doxycycline in the presence of 10nM R1881 or ethanol vehicle for 24 hours.
|
| Growth protocol |
AR10Q, AR112Q, and AR112QKRKR PC12 cells were cultured at 37ºC at 10% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the Ambion WT kit protocol from 250 ng total RNA (GeneAtlas™ WT Expression Kit User Manual P/N 702935 Rev. 3).
|
| |
|
| Hybridization protocol |
Following fragmentation, 3.7 ug of cDNA were hybridized for 20 hr at 48C on Mouse Gene 2.1 ST Array Strips using the Affymetrix Gene Atlas system (software version 1.0.4.267).
|
| Scan protocol |
Arrays were scanned using the Affymetrix Gene Atlas system
|
| Description |
Gene expression data from PC12 cells expression AR112Q-KRKR in the presence of androgen
|
| Data processing |
Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical environment 3.0.0. RMA was used to normalize the data and fit log2 transformed expression values.
|
| |
|
| Submission date |
Aug 27, 2013 |
| Last update date |
Aug 23, 2014 |
| Contact name |
Andrew Lieberman |
| E-mail(s) |
liebermn@med.umich.edu
|
| Organization name |
University of Michigan
|
| Department |
Pathology
|
| Street address |
3510 MSRB I 1150 W Medical Center Dr
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL17400 |
| Series (1) |
| GSE50383 |
AR function is altered by polyglutamine expansion and by SUMO |
|
