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Sample GSM1220300

Query DataSets for GSM1220300

Status

Public on Dec 12, 2013

Title

Reference Epigenome: ChIP-Seq Input from Human Psoas Muscle Tissue; renlab.Input.STL003PO.02.01

Sample type

SRA

Source name

Psoas Muscle tissue; renlab.Input.STL003PO.02.01

Organism

Homo sapiens

Characteristics

sra sample accession: SRS306621
disease: None
biomaterial_provider: Shin Lin, Stanford University
biomaterial_type: Primary Tissue
tissue_type: Psoas Muscle
tissue_depot: N/A
collection_method: Autopsy
donor_id: STL003
donor_age: 34
donor_health_status: polysubstance abuse (NO diabetes, hypertension, coronary artery disease, cancer)
donor_sex: Male
donor_ethnicity: Caucasian
experiment_type: ChIP-Seq Input
extraction_protocol: See http://bioinformatics-renlab.ucsd.edu/RenLabChipProtocolV1.pdf
extraction_protocol_type_of_sonicator: Biorupter
extraction_protocol_sonication_cycles: 80
chip_protocol: Input
chip_protocol_chromatin_amount: 500 micrograms

Extracted molecule

genomic DNA

Extraction protocol

Library construction protocol: See http://bioinformatics-renlab.ucsd.edu/RenLabLibraryProtocolV1.pdf

Library strategy

ChIP-Seq

Library source

genomic

Library selection

RANDOM

Instrument model

Illumina HiSeq 2500