Growth conditions as described in Harris, A., and Coleman, L. (1989) Ductal epithelial cells cultured from human fetal epididymis and vas deferens: relevance to sterility in Cystic Fibrosis. J.Cell Sci., 92: 687-690.
Extracted molecule
total RNA
Extraction protocol
RNA was isolated by Trizol extraction from the same three primary cultures of HEE (Human Epididymis Epithelial) cells that were used for DNase-seq and at the same timepoint. Total RNA was purified by Millipore Microcon YM-100 filter centrifugation.
Label
Cy3
Label protocol
Labeling was performed by MOGene, LC, St. Louis, MO, USA following their standard operating protocol. See www.mogene.com.
Hybridization protocol
Hybridization was performed by MOGene, LC, St. Louis, MO, USA following their standard operating protocol. See www.mogene.com.
Scan protocol
Scanning was performed by MOGene, LC, St. Louis, MO, USA following their standard operating protocol. See www.mogene.com.
Description
Expression values for human genes in HEE cells
Data processing
Data were processed by MOGene, LC, St. Louis, MO, USA following their standard operating protocol. See www.mogene.com.
A genome-wide analysis of open chromatin in human epididymis epithelial cells reveals novel candidate regulatory elements for male infertility [Transcriptome data]
