|
| Status |
Public on Jul 27, 2006 |
| Title |
Drosophila_tolerance_5 |
| Sample type |
RNA |
| |
|
| Source name |
whole body flies from a population that had developed tolerance 2h after the initial ethanol exposure
|
| Organism |
Drosophila melanogaster |
| Characteristics |
3-5 day old male Canton S flies from a population that had developed tolerance 2h after the initial ethanol exposure.
|
| Treatment protocol |
flies exposed to ethanol twice with 2 h interval
|
| Growth protocol |
flies exposed to ethanol twice with 2 h interval
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions
|
| Label |
Biotinylated cRNA
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 mkg of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Overall design: We collected 3-5 day old Canton S flies either without exposure to ethanol (control group), immediately after passage through the inebriometer, or from a population that had developed tolerance 2h after the initial ethanol exposure.
|
| Data processing |
scale factor 2.575
|
| |
|
| Submission date |
Jul 24, 2006 |
| Last update date |
Jul 26, 2006 |
| Contact name |
Tatiana V Morozova |
| E-mail(s) |
tvmorozo@ncsu.edu
|
| Organization name |
NCSU
|
| Department |
zoology
|
| Lab |
Anholt
|
| Street address |
campus box 7617
|
| City |
Raleigh |
| State/province |
NC |
| ZIP/Postal code |
27695 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE5382 |
Transcriptional Response to Alcohol Exposure in Drosophila melanogaster |
|
