|
| Status |
Public on Feb 15, 2016 |
| Title |
Heart, Infarcted, rep6 |
| Sample type |
RNA |
| |
|
| Source name |
Heart, Infarcted
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
tissue: heart
age: 12 week-old
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Trizol (Invitrogen). RNA quantities and quality were assessed using a NanoDrop ND-1000 spectrophotometer or an Agilent 2100 BioanalyzerRNA quantities and quality were assessed using a NanoDrop ND-1000 spectrophotometer or an Agilent 2100 Bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
200ng of total RNA were used to perform target preparation using the WT Expression Kit (Life Technologies). 5.5 μg of each fragmented cDNA was end-labeled with biotin
|
| |
|
| Hybridization protocol |
Arrays (Affymetrix, Santa Clara , CA, USA) were hybridized with 2.7μg of biotinylated target, at 45°C for 17 hours washed and stained according to the protocol described in Affymetrix GeneChip® Expression Analysis Manual (Fluidics protocol FS450_0007).
|
| Scan protocol |
Arrays were scanned using the GeneChip® Scanner 3000 7G (Affymetrix)
|
| Description |
12 week old mice, border zone of infarct, 2 weeks after surgery
|
| Data processing |
Background subtraction, quantile normalization and probeset summarization was performed using the Affymetrix Power Tools apt-probeset-summarize command with the -rma option
|
| |
|
| Submission date |
Sep 19, 2013 |
| Last update date |
Feb 16, 2016 |
| Contact name |
Mark Ibberson |
| Organization name |
SIB Swiss Institute of Bioinformatics
|
| Department |
Vital-IT
|
| Street address |
Genopode building
|
| City |
Lausanne |
| ZIP/Postal code |
CH-1015 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL10740 |
| Series (2) |
| GSE51012 |
Comparative transcriptome profiling of the injured zebrafish and mouse hearts identifies miRNA-dependent repair pathways |
| GSE51014 |
Comparative transcriptome profiling of the injured zebrafish and mouse hearts identifies miRNA-dependent repair pathways |
|
