|
| Status |
Public on Sep 30, 2014 |
| Title |
LN229-SOX4 |
| Sample type |
RNA |
| |
|
| Source name |
LN229_SOX4 overexpression
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: LN229
cell type: glioblastoma cells
expressing: pBrit-Flag-SOX4-HA
genotype/variation: SOX4 overexpression
|
| Treatment protocol |
SOX4 was overexpressed with pBrit-Flag-SOX4-HA or pBrit-Flag-HA in LN229
|
| Growth protocol |
cells were cultured in DMEM with 10% FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted with Trizol according to the manufacturer's protocol
|
| Label |
biotin
|
| Label protocol |
Start with 250ng total RNA, and Biotinylated cRNA were prepared according to the standard Affymetrix protocol, in which, GeneChip 3 IVT Express Kit (Lot no.901228) is used in the experiment.
|
| |
|
| Hybridization protocol |
Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hours at 45 ℃ onPrimeView Human Gene Expression Array. GeneChips were washed and stained in the Affymetrix fluidics Station 450.
|
| Scan protocol |
The chips were scanned with the affymetrix genechip 7G plus, and AGCC is used for the image processing
|
| Description |
SOX4 overexpression LN229
|
| Data processing |
Expression Console is used for normalizing the chip data and analyzing the fold change
|
| |
|
| Submission date |
Sep 30, 2013 |
| Last update date |
Sep 30, 2014 |
| Contact name |
jie liu |
| E-mail(s) |
liujie_1914@163.com
|
| Organization name |
zcni
|
| Street address |
yuhangtang road 866
|
| City |
hangzhou |
| ZIP/Postal code |
310058 |
| Country |
China |
| |
|
| Platform ID |
GPL15207 |
| Series (1) |
| GSE51301 |
Genes regulated by SOX4 in glioblastoma cell line |
|
