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Sample GSM124520 Query DataSets for GSM124520
Status Public on Aug 02, 2006
Title embryo at 4-6hour, fraction 1-5,EDTA treated biological rep1
Sample type RNA
 
Source name Drosophila embryos
Organism Drosophila melanogaster
Characteristics Canon S embryos
Treatment protocol embryos were collected in 2-hour intervals and aged to generate animals 0-2, 4-6 and 8-10 hour old.
Extracted molecule total RNA
Extraction protocol EDTA-treated embryos were lysed in an EDTA extaction buffer (20mM Tris, pH7.4, 140mM KCl, 15mM EDTA, 0.5mM DTT, 1% Triton X-100, 0.1mg/ml cycloheximide, 1mg/ml heparin, 50unit/ml RNasin) and sedimented through 20-50% gradients prepared with the same EDTA lysis buffer, but without Triton X-100.
Label biotin
 
Hybridization protocol Following fragmentation, at least 10 microg of cRNA were hybridized on GeneChip Drosophila Genome 2.0 Array.
Description Gene expression data from embryos during first 10 hours after egg laying to see the global and transcript-specific translation profile
Data processing Gene expression measures were computed using the Robust Multichip Average (RMA) method and implemented in the Bioconductor R package.
 
Submission date Aug 01, 2006
Last update date Aug 28, 2018
Contact name Soyeon Ahn
E-mail(s) ahn@stat.berkeley.edu
Organization name UC Berkeley
Street address 367 Evans Hall #3860
City Berkeley
State/province CA
ZIP/Postal code 94720
Country USA
 
Platform ID GPL1322
Series (1)
GSE5430 Expression data from early Drosophila embryo
Relations
Reanalyzed by GSE119084

Data table header descriptions
ID_REF
VALUE intensity after RMA

Data table
ID_REF VALUE
1616608_a_at 8.900923672
1622892_s_at 8.86779978
1622893_at 3.012334354
1622894_at 4.59593297
1622895_at 9.271336438
1622896_at 4.240135858
1622897_at 8.137552692
1622898_a_at 9.954415672
1622899_at 4.195241125
1622900_at 3.250939658
1622901_at 3.756392726
1622902_at 4.467379474
1622903_s_at 7.964977672
1622904_at 4.984421109
1622905_at 3.880127217
1622906_at 5.780711481
1622907_at 8.140065626
1622908_a_at 5.260444488
1622909_at 6.799334835
1622910_at 3.708008992

Total number of rows: 18952

Table truncated, full table size 432 Kbytes.




Supplementary file Size Download File type/resource
GSM124520.CEL.gz 3.4 Mb (ftp)(http) CEL

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