|
| Status |
Public on Jun 09, 2015 |
| Title |
5-Fluoro-uracil_2 |
| Sample type |
RNA |
| |
|
| Source name |
ATCC
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Colon adenocarcinoma
sample type: HT29 xenograft
treatment: 5-Fluoro-uracil
|
| Treatment protocol |
Mice were treated for 3 days and sacrificed 24h after treatments end.
|
| Growth protocol |
Tumor cells from HT29 cell line were subcutaneously injected in NOD/SCID mice, and tumors grew for 14 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted according to manufactures' guidelines for QiaCube device (Qiagen).
|
| Label |
biotin
|
| Label protocol |
100ng of total RNA were amplified and labeled with the IVT Express Kit (Affymetrix); all steps were performed according to the manufacturers' protocols.
|
| |
|
| Hybridization protocol |
130µl hybridization mix prepared from labeled cRNA fragments was hybridized for 16h at 45°C and 60 rpm to GeneChip PrimeView arrays. Microarrays were washed and stained in the Affymetrix Fluidics Station 450 using script FS450_0002.
|
| Scan protocol |
Microarrays were scanned using a GeneChip Scanner 3000 7G (Affymetrix).
|
| Description |
HT29 xenograft with 5-Fluoro-uracil treatment, biological replicate 2
|
| Data processing |
Data were preprocessed in the R v3.0.1 environment with Bioconductor packages affy and vsn; VSN normalization and RMA probeset summary were performed with the vsnrma command using default parameter settings.
|
| |
|
| Submission date |
Oct 30, 2013 |
| Last update date |
Jun 09, 2015 |
| Contact name |
Claus Juergen Scholz |
| Organization name |
University Hospital Würzburg
|
| Department |
Core Unit SysMed
|
| Street address |
Josef-Schneider-Str. 2
|
| City |
Würzburg |
| ZIP/Postal code |
97080 |
| Country |
Germany |
| |
|
| Platform ID |
GPL15207 |
| Series (1) |
| GSE51904 |
The antidepressant fluoxetine elicits anticancer effects by impairing aerobic glycolysis in colon cancer cells and xenografts. |
|
