|
Status |
Public on Jan 30, 2014 |
Title |
GFP Zfx ko 1 |
Sample type |
RNA |
|
|
Source name |
Sorted Zfx ko myeloid progenitors overexpressing only GFP selectable marker
|
Organism |
Mus musculus |
Characteristics |
genotype/variation: Zfx fl/y CreER treatment: infected with control retrovirus with GFP selectable marker
|
Treatment protocol |
Tamoxifen was administered via 5 mg daily gavage for three days.
|
Growth protocol |
Cells were cultured in RPMI 1640 with 20% FBS and the following cytokines: IL-3 (10 ng/mL), IL-6 (10 ng/mL), SCF (10 ng/mL)
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Used the Ambion WT labelling kit as per manufacturer's instructions using 200ng of starting total RNA
|
|
|
Hybridization protocol |
Biotinylated DNA probe was made according to the standard Affymetrix protocol
|
Scan protocol |
Arrays were scanned according to the Affymetrix protocol
|
Data processing |
Gene level RMA
|
|
|
Submission date |
Nov 15, 2013 |
Last update date |
Jan 30, 2014 |
Contact name |
Stuart Weisberg |
E-mail(s) |
spw13@cumc.columbia.edu
|
Organization name |
Columbia University
|
Department |
Pathology and Cell Biology
|
Lab |
Weisberg
|
Street address |
650 W 168th Street
|
City |
New York |
State/province |
NY |
ZIP/Postal code |
10032 |
Country |
USA |
|
|
Platform ID |
GPL6246 |
Series (2) |
GSE43022 |
ZFX controls the propagation and cell-of-origin characteristics of acute leukemia |
GSE52416 |
Effect of genetic Zfx deletion on Myc induced gene expression in bone marrrow myeloid progenitors |
|