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Sample GSM1265780 Query DataSets for GSM1265780
Status Public on Jan 30, 2014
Title Myc Zfx wt 2
Sample type RNA
 
Source name Sorted Zfx wt myeloid progenitors overexpressing Myc using GFP selectable marker
Organism Mus musculus
Characteristics genotype/variation: Zfx wt/y CreER
treatment: infected with Myc expressing and control retrovirus with GFP selectable marker
Treatment protocol Tamoxifen was administered via 5 mg daily gavage for three days.
Growth protocol Cells were cultured in RPMI 1640 with 20% FBS and the following cytokines: IL-3 (10 ng/mL), IL-6 (10 ng/mL), SCF (10 ng/mL)
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Used the Ambion WT labelling kit as per manufacturer's instructions using 200ng of starting total RNA
 
Hybridization protocol Biotinylated DNA probe was made according to the standard Affymetrix protocol
Scan protocol Arrays were scanned according to the Affymetrix protocol
Data processing Gene level RMA
 
Submission date Nov 15, 2013
Last update date Jan 30, 2014
Contact name Stuart Weisberg
E-mail(s) spw13@cumc.columbia.edu
Organization name Columbia University
Department Pathology and Cell Biology
Lab Weisberg
Street address 650 W 168th Street
City New York
State/province NY
ZIP/Postal code 10032
Country USA
 
Platform ID GPL6246
Series (2)
GSE43022 ZFX controls the propagation and cell-of-origin characteristics of acute leukemia
GSE52416 Effect of genetic Zfx deletion on Myc induced gene expression in bone marrrow myeloid progenitors

Data table header descriptions
ID_REF
VALUE RMA linear signal

Data table
ID_REF VALUE
10344614 148.4369
10344616 24.21
10344620 41.42569
10344622 359.8807
10344624 3059.752
10344633 2324.988
10344637 1046.76
10344653 34.33128
10344658 570.293
10344674 38.00885
10344679 73.63183
10344707 438.7914
10344713 3841.547
10344715 44.73404
10344717 33.29292
10344719 124.6269
10344721 21.87057
10344723 440.8478
10344725 196.9025
10344741 4451.204

Total number of rows: 35556

Table truncated, full table size 621 Kbytes.




Supplementary file Size Download File type/resource
GSM1265780_9.CEL.gz 4.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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