|
| Status |
Public on Jan 30, 2014 |
| Title |
GFP Zfx wt 2 |
| Sample type |
RNA |
| |
|
| Source name |
Sorted Zfx wt myeloid progenitors overexpressing only GFP selectable marker
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: Zfx wt/y CreER
treatment: infected with control retrovirus with GFP selectable marker
|
| Treatment protocol |
Tamoxifen was administered via 5 mg daily gavage for three days.
|
| Growth protocol |
Cells were cultured in RPMI 1640 with 20% FBS and the following cytokines: IL-3 (10 ng/mL), IL-6 (10 ng/mL), SCF (10 ng/mL)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Used the Ambion WT labelling kit as per manufacturer's instructions using 200ng of starting total RNA
|
| |
|
| Hybridization protocol |
Biotinylated DNA probe was made according to the standard Affymetrix protocol
|
| Scan protocol |
Arrays were scanned according to the Affymetrix protocol
|
| Data processing |
Gene level RMA
|
| |
|
| Submission date |
Nov 15, 2013 |
| Last update date |
Jan 30, 2014 |
| Contact name |
Stuart Weisberg |
| E-mail(s) |
spw13@cumc.columbia.edu
|
| Organization name |
Columbia University
|
| Department |
Pathology and Cell Biology
|
| Lab |
Weisberg
|
| Street address |
650 W 168th Street
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE43022 |
ZFX controls the propagation and cell-of-origin characteristics of acute leukemia |
| GSE52416 |
Effect of genetic Zfx deletion on Myc induced gene expression in bone marrrow myeloid progenitors |
|
