|
Status |
Public on Mar 30, 2015 |
Title |
Untransfected cells 0h rep 1 (SETX) |
Sample type |
RNA |
|
|
Source name |
A549 cells
|
Organism |
Homo sapiens |
Characteristics |
treatment: no siRNA infection: no infection cell line: A549
|
Treatment protocol |
A549 cells were transfected with either control siRNA (D-001810-10-05, Dharmacon) or SETX siRNA (23064, Dharmacon) pools using the Lipofectamine™ RNAiMAX Transfection Reagent (Invitrogen). Transfections were performed according to the manufacturer’s instructions. 48 hours post transfection, the cells were infected with the Influenza A/Puerto Rico/8/34(ΔNS1) strain at an multiplicity of infection of 3. Wells of uninfected cells were used as controls for infection. Cells were collected for further processing 4 hours post infection. RNA and protein levels of SETX were quantified to determine knockdown efficiency.
|
Growth protocol |
Standard culture techniques
|
Extracted molecule |
total RNA |
Extraction protocol |
QIAGEN Rneasy mini kit
|
Label |
biotin
|
Label protocol |
Biotin-labeled RNA was prepared with MessageAmp™ Premier RNA Amplification Kit (Applied Biosystems)
|
|
|
Hybridization protocol |
Standard Illumina protocol
|
Scan protocol |
Standard Illumina protocol
|
Description |
Replicate 1
|
Data processing |
Data was analysed using Genespring GX12.5 . To analyze gene expression changes during stimulation, raw data were subject to quantile normalization and baseline transformation was performed to the median of unstimulated samples of the corresponding siRNA treatment.
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|
|
Submission date |
Dec 03, 2013 |
Last update date |
Mar 30, 2015 |
Contact name |
Ivan Marazzi |
Organization name |
Icahn School of Medicine at Mount Sinai
|
Department |
Department of Microbiology
|
Street address |
1468 Madison Avenue
|
City |
New York |
ZIP/Postal code |
10029 |
Country |
USA |
|
|
Platform ID |
GPL10558 |
Series (2) |
GSE52932 |
Analysis of global gene expression profiles of A549 cells treated with SETX siRNA upon Influenza A/PR/8/34(ΔNS1) Infection |
GSE52937 |
Senataxin suppresses the antiviral transcriptional response and controls viral biogenesis |
|
Relations |
Reanalyzed by |
GSM1278321 |